研究意義:

細(xì)胞中，細(xì)胞色素P450主要分布在內(nèi)質(zhì)網(wǎng)和線粒體內(nèi)膜上，作為一種末端加氧酶，參與了生物體內(nèi)的甾醇類激素合成等過(guò)程。近年來(lái)，對(duì)細(xì)胞色素P450的結(jié)構(gòu)、功能特別是對(duì)其在藥物代謝中的作用的研究有了較大的進(jìn)展。研究表明細(xì)胞色素P450是藥物代謝過(guò)程中的關(guān)鍵酶，而且對(duì)細(xì)胞因子和體溫調(diào)節(jié)都有重要影響。

相關(guān)文獻(xiàn):

【文獻(xiàn)標(biāo)題】Enantioselective toxicity ａnd oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway

【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al

【作者單位】中國(guó)農(nóng)業(yè)科學(xué)院煙草研究所（Tobacco Research Institute of Chinese Academy of Agricultural Sciences）

【文獻(xiàn)中引用產(chǎn)品】

蚯蚓8-羥基脫氧鳥(niǎo)苷（8-OHdG）ELISA試劑盒

蚯蚓細(xì)胞色素P450(CYP450)ELISA試劑盒

蚯蚓羥基自由基(OH)ELISA檢測(cè)試劑盒

【關(guān)鍵詞】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic

【DOI】doi.org/10.1016/j.scitotenv.2020.142630

【影響因子(IF)】8.6

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

The OH content, 8-OhdG content, ａnd CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, ａnd activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at 450 nm. The detection principle ａnd method of 8-OhdG ａnd CYP450 are the same as those of OH.

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